Genetics

RNA Processing

1. IntraNuclear
   • 5’ capping
   • poly-A tail addition
   • intron splicing
2. IntraCellular
   1. Translation–> associate with Ribosomes
   2. Storage & Regulation –> associte with proteins found in P-bodies
   3. Poly-A tail is gradually shortened—> degradation

Cell Division Phases

• G0: resting
• Interphase -
  1. G1 (RNA/Protein/lipid/carp synthesis)
  2. S (DNA synthesis)
  3. G2 (ATP syn)
• M phase (Cell division)

Tumor Suppressor Gene: RB1

• rb protein binds and inhibits E2F transcription factor –> prevent G1/S transition
• When ready –> Rb protein phosphorylated by cyclin depdendent kinases –> E2F is uninhibited –> cell division
• Loss of function –> retinoblastoma, osteosarcoma